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BioProtein+Plus - Biologically Active Thymus Proteins plus Zinc

Balances Immune System Function

By, Daniel Clark M.D.

Immune Dysfunction

Dysfunction of the Immune System affects about 65 million Americans. These dysfunctions manifest themselves in disorders such as Chronic Allergies and Respiratory problems, Arthritis, Diabetes, Lupus Erythematosus and Chronic Viral infections to name a few. The cost in terms of lost time at work, cost of related health care and diminished quality of life cannot be accurately estimated. However, everyone agrees that the costs are enormous. A good deal of the time and money related to Immune Dysfunction is actually spent treating the disorders rather than directly approaching the underlying cause itself.

Immune Dysfunction is likely the result of a combination of assaults on the immune system. Generally there is immune suppression then infection followed by treatment of the infection. If immune function is not restored, the initial infection may not resolve, or it may be followed by another infection eventually overwhelming the immune system causing it to overreact and under-react simultaneously. This is classic Immune Dysfunction, a patient with multiple low-grade infections and autoimmune disorders at the same time. Direct immune stimulation may exacerbate the autoimmune disorders, while treating autoimmune disorders may suppress immunity and allow infection to proliferate.

The Thymus Gland

The “brain” of the immune system is the thymus gland. It lies just beneath the breastbone and is responsible for producing perhaps dozens of different polypeptides that “program” lymphocyte progenitors. Lymphocytes are born in the bone marrow; some receive their programming in the bone marrow and are referred to as B-lymphocytes. Others migrate to the thymus gland to mature by contacting the thymic proteins and are referred to as T-lymphocytes. The T-lymphocytes display receptor sites on their surface that correspond to transmitter sites on the surface of the various thymic proteins. When the appropriate T-lymphocyte contacts its corresponding protein, the transmitter fits into the receptor (much like a key fits into a lock) and the lymphocyte is now ready to perform its specific function or is otherwise immune-competent.

There are two main types of T-lymphocytes. These are the T-4 (CD4) helper, and the T-8 (CD8) that further differentiate into killer and suppressor types. Helper cells not only induce killer and suppressor cells but also induce B-lymphocytes, and macrophages. Killer cells do just that, kill. When they are immune-competent they have the ability to recognize deviant cells such as tumor cells and the body’s own cells that are virally infected. Once recognized, the killer cell attacks. Sometimes killer cells overreact by attacking self-tissue (auto-immune disease) but if immune-competent suppressor cells are present, they intervene and stop the attack. Thus when the system is functioning properly the thymus produces proteins that “program” T-lymphocytes that start and stop immune response.

Due to genetic flaw, trauma, stress, disease, exposure to toxic substances and/or inevitably the aging process, the thymus ceases to produce its proteins in the proper quantity or combinations to maintain immune balance.

Supplementation with Thymic Proteins

The use of thymic extracts has been well studied. There is a substantial body of clinical evidence that indicates the use of thymus is effective in the treatment of a number of diseases and disorders. Chronic viral infections, different types of cancers, and some auto-immune disorders are a few that I will discuss here.

Double-blind studies reveal that thymus extracts are able to eliminate viral infection; and continued use over one year reduces the number of recurrent infections. Additionally, patients in the studies show improved immune parameters1. In cancer patients, the primary indication for thymus use is for the treatment of lymphopenia/leukopenia caused by chemotherapy and radiation treatments. The effect is to prevent or at least mitigate the dramatic drop in white blood cell levels and to restore counts more quickly in cases where they do drop. The net result of patients taking thymus are that they are more often able to complete their treatment and with reduced side effects than in patients who do not take thymus2,3. Autoimmune disorders are immune dysfunction where the bodies’ own defense mechanisms are attacking self-tissue.

Rheumatoid Arthritis, Lupus, and Scleroderma are classic disorders caused by increased antibody formation brought on by a high T-helper/T-suppressor ratio. A clinical study in Rheumatoid Arthritis patients revealed that thymus extract use normalized their T-helper/T-suppressorratios in three months2. Thymus is also indicated or the treatment of Hepatitis C, AIDS, Chronic Fatigue, Cold and Flu, Lupus Erythematosis, Type II Diabetes and Scleroderma. Studies are presently being conducted for the treatment of Hepatitis C.

Another area of recent study for the application of thymus products has to do with the role lymphocytes play in periodontal disease. The first line of defense against bacterial plaque is neutrophils. Neutrophils are depressed in people susceptible to periodontal disease such as diabetics and the geriatric population. Lymphocytes promote neutrophil growth and thymus proteins promote lymphocyte growth. Lymphocytes therefore play a major role in prevention and resolution of periodontal disease10,11.

Volumes of scientific data exist now to support the use of thymus products for immune suppression and immune dysfunction. There is precious little data however suggesting which specific thymus product is most effective.

Which Thymus Products are Best?

There are a variety of thymus products available from raw thymus tissue to highly purified thymus proteins and many thymus extracts in between. Thymus proteins are produced in the thymic epithelium and range in size from probably around 10,000 Dalton to perhaps over 50,000 Dalton. Whether large or small, proteins are complex molecules that can be easily damaged (de-natured) or changed. Changes in the protein molecule can bear directly on its biological activity and therefore its ability to “program” T-lymphocytes. Therefore the manner in which the thymus product is handled and administered will bear directly upon howmuch benefit the patient experiences from using it.

Typical thymic extracts are composed of ground up thymus tissue, which is then freeze-dried and encapsulated to be swallowed and absorbed in the gastrointestinal tract. There are also liquid thymus extract drops that are taken sublingually and a few products of freeze-dried purified thymus protein that is also administered sublingually.

My experience has taught me that the purified thymus protein administered sublingually is the most effective. I have recently been testing a thymic compound called BioProtein+PlusTM that contains purified biologically active thymus proteins ranging in atomic size from 10,000 to 50,000 Dalton plus Zinc Gluconate.

The Role of Zinc

DNA makes RNA, which in turn makes proteins. Proteins drive biological function. Trace minerals work as catalysts or activators in enzyme complexes and influence reactivity and stability of protein configurations. Zinc has anti-oxidant properties and serves a vital role in many immune functions. Studies have shown that Zinc must be present for lymphocyte maturation and is a lymphocyte mitogen. Additionally, zinc is a necessary co-factor in activation of serum thymic factor5,6. Zinc Gluconate has been clinically proven to reduce the duration of the common cold by an average of 7 days7,8.

Zinc may also have some anti-aging benefits. An important study of zinc supplementation in elderly patients resulted in increased numbers of T-cells and enhanced cell-mediated immune responses9.

Our testing indicates that BioProtein+PlusTM works to regulate the T-helper/T-killer/T-suppressor interaction resulting in normalization of immune response. Our experience is consistent with clinical studies conducted with other thymus products. The quality of our results are due to the combination of biologically active purified thymus proteins contained in BioProtein+PlusTM working synergistically with the zinc gluconate to induce immune regulation.

A Unique Thymic Compound

BioProtein+PlusTM is a proprietary formula produced by the original manufacturer of BioPro Thymic Protein A. The thymus used in its manufacture comes from dairies in the US that only produce certified raw dairy products. It is certified free of microorganisms and BSE. The proteins are purified efficiently by the most up to date assay techniques while taking great care to maintain the proteins’ biological activity. They are then lyophilized to maintain their long-term stability.

The zinc gluconate used in the manufacture of BioProtein+PlusTM is of the highest quality available. Once freeze-dried, the purified thymus proteins and the zinc gluconate are triturated into a base of maltodextrin and packaged in doses containing about 400 mg of product each.

It is recommended by the manufacturer to be taken as a once per day supplement. Just as with BioPro Thymic Protein A, we have not seen any adverse side effects. On some occasions a health care professional may recommend more than one per day. As with other zinc products, toxicity does not occur unless a person takes over 150 mg of zinc per day for more than two weeks. BioProtein+PlusTM contains 10 mg of zinc per dose.

There are no known contraindications but BioProtein+PlusTM is likely to be less effective for people using steroidal medications.

Clinical Observations

One completed trial has been conducted on patients infected with HIV and resistant to antiretroviral treatment. It concluded that BioProtein+PlusTM increased CD4 cell counts without side effects.

The study was conducted in a pediatric hospital in Vietnam. There were 60 children enrolled, 30 in the study group receiving 2 packets per day and 30 in a control group. The average increase in CD4 count over the 30 day study was almost double without liver toxcicity or other side effects.12

Hepatitis C

Twenty-seven people completed the trial that measured ALT, AST, and total viral load over six months. Twenty-two of the participants experienced statistically significant reductions in ALT, AST, and RNA PCR. No one reported any negative side effects.

Chemotherapy Toxicity

Ethel began Taxol treatment for breast cancer in December 1999. She began using three packets of BioProtein+Plus TM per day when she began treatment. She has received 8 rounds of Taxol. Throughout her treatment her blood parameters have remained in the normal range except her lymphocyte count dropped below normal for six weeks from mid-March to the end of April. She completed her Taxol treatment without interruption.

Brief reports from health care professionals:

Kathryn, an acupuncturist in the state of Florida, has found the new BioProtein+Plus to be very effective at reducing viral load on her Hepatitis C patients referred to her by an Internist for acupuncture treatments.

Michael, an acupuncturist also in the state of Florida, has found the new BioProtein+Plus effective in balancing immune function for a number of patients with autoimmune disorders.

Denise and Luby Chambul, D.C. of Nashville, Tennessee have used the BioProtein+Plus in Hepatitis C patients with effective reduction of viral load.

Dr. Herbert Warren of Alternative Health Concepts reports: I have been dispensing BioPro Thymic Protein A for over 4 years, to clients with a variety of health problems ranging form simple immune dysfunction to severe debilitating diseases like; Chronic Fatigue, Hepatitis C, severe Lymphopenia, and Cancer. In the vast majority of cases (90%), I have seen positive health benefits when the product was taken as recommended.

Since being introduced to the “new generation” product, BioProtein+Plus, I have observed the same extraordinary health benefits previously experienced with the aforementioned product and in several cases a more natural or complete immune regulation response has been observed, with out the “inflammatory flare” sometimes seen with BioPro. I can only surmise this is because of the more balanced and complete formulation of purified thymus proteins coupled with the zinc found in BioProtein+Plus.

I feel both products are phenomenal in enhancing the immune response, however, because of the smooth immune modulation and the cost differential between the two products, I prefer BioProtein+Plus, as the supplement of choice to fully restore the cell mediated immunity so necessary for a healthy life.


Nothing teaches and convinces like one’s own experience. The author’s own observations and experience as well as other health care professionals is that BioProtein+PlusTM is an excellent tool in the battle against immune dysfunction.

If you are a person who suffers with chronic viral infection while at the same time dealing with autoimmune disorder, ask your health professional about this product. If you are a health professional looking for an effective and cost-effective tool to recommend to your clients, consider trying this product and experience for yourself the benefits of this product.


Riocchi A, Borella E, Riva E. et al. A double blind clinical trial for the evaluation of the therapeutic effectiveness of a calf thymus derivative in children with recurrent repiratory infection. Thymus 1986; 8: 831-839.

Kouttab NM, Prada M., Cazzola P. Thymomodulim. Biological properties and clinical applications. Med Oncol Tumor Pharmacother 1989; 6: 5-9.

Kang SD, Lee B H, Yand JH, Lee CY. The effects of calf-thymus extract on recovery of bone marrow function in anticancer chemotherapy. New Med J (Korea) 1985; 28: 11-15.

Cazzola P, Mazzanti P, Bossi G. In vivo modulation effect of a calf thymus acid lysate on human T Lymphocyte subsets and CD4+/CD8+ ratio in the course of different diseases. Curr Ther Res 1987; 42: 1011-1017.

Eaterbrook-Smith S. Activation of the binding of C1q to immune complexes by zinc. FEBS Lett 1983; 162: 117-119.

Hadden JW. The treatment of zinc deficiency is an immunotherapy. Int J Immunopharmac 1995; 17: 697-701.

Eby GA, Davis DR, Halcomb WW. Reduction in duration of common colds by zinc gluconate lozenges in a double-blind study. Antimicrob Agents Chemother 1984; 25: 20-24.

Mossad SB, Macnin ML, Medendorp SV et al. Zinc gluconate lozenges for treating the common cold. A randomized, double blind, placebo-controlled study. Annals Intern Med 1996; 125: 81-88.

Gershwin M, beach R, Hurley L. Trace metals, aging and immunity. J Am Ger Soc 1983; 31: 374-378.

Carranza F. Glickman’s clinical peridontology. Philadelphia, PA: WB Saunders. 1984.

Page R, Schroeder H. Current status of the host response in chronic marginal periodontis. J Periodontal 1981; 53: 477-491

Khu thi Khanh Du., Nguyen Van Lam & Associate, Assessment of BioProtein Plus Effectiveness of increasing CD4 concentration of HIV patients, 2010.

About the Author:

Dr. Daniel Clark is a noted researcher and lecturer in the field of alternative medicines.